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Magnetic Separation Of Cells In Blood

Abstract Direct magnetic separations of red blood cells from whole blood have been carried out using a continuous magnetic separation method based on high gradient magnetic separation HGMS and a gaspermeable membrane with nitrogen gas The experimental results have shown good agreements with the theoretical model taking into account the gravitational force

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  • Continuous magnetic separation of blood components from

    Abstract Direct magnetic separations of red blood cells from whole blood have been carried out using a continuous magnetic separation method based on high gradient magnetic separation HGMS and a gaspermeable membrane with nitrogen gas The experimental results have shown good agreements with the theoretical model taking into account the gravitational force

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  • Magnetic separation of malariainfected red blood cells in

    Abstract Malaria is a serious disease that threatens the public health especially in developing countries Various methods have been developed to separate malariainfected red blood cells iRBCs from blood samples for clinical diagnosis and biological and epidemiological research In this study we propose a simple and labelfree method for separating not only latestage but also earlystage iRBCs on the basis of their paramagnetic characteristics due to the malaria

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  • Detection of tumor cells in blood using CD45 magnetic cell

    Aug 18 2000 A new method for detecting circulating tumor cells that is based on magneticactivated cell separation MACS and nested mutant allelespecific amplification nested MASA was evaluated in patients with colorectal cancer using the p53 and Kras genes as genetic markersBy negative selection with antiCD45 monoclonal antibodyconjugated supermagnetic microbeads the proportion of

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  • Removal of Cells from Body Fluids by Magnetic Separation

    Aug 22 2017 The magnetic separation of pathogenic compounds from body fluids is an appealing therapeutic concept Recently removal of a diverse array of pathogens has been demonstrated using extracorporeal dialysistype devices The contact time between the fluid and the magnetic beads in such devices is limited to a few minutes This poses challenges particularly if large compounds such as

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  • Cell Separation and Cell Isolation Techniques Methods

    Cell separation also commonly referred to as cell isolation or cell sorting is a process to isolate one or more specific cell populations from a heterogeneous mixture of cells There are a number of cell separation methods available each with its own pros and cons

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  • Magnetic Bead Cell Separation ProImmune Mastering

    Cell Separation using Pro5 Pentamers and Magnetic Beads In addition to detecting antigenspecific T cells Pro5 MHC Class I Pentamers can be used in conjunction with magnetic beads to enrich for the T cell population of interest Magnetic bead sorting is a simple solution for applications requiring enrichment of antigenspecific T cells for example therapeutic

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  • Ultrahighthroughput magnetic sorting of large blood

    CTCs by depleting RBCs platelets and white blood cells WBCs in a tumoragnostic manner The LPCTCiChip consists of inertial separation array devices for removal of RBCs and platelets followed by a highgradient magnetic cell sorter for the depletion of WBCs The development of this ultrahigh

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  • Immunomagnetic Bead Separation of Mononuclear Cells

    Density gradient centrifugation usually allows efficient separation of mononuclear cells from granulocytes using fresh human blood samples However we have found that with cryopreserved blood samples density gradient centrifugation fails to separate granulocytes from mononuclear cells and have explored using immunomagnetic antiCD15 microbeads as an alternate method to separate these cell

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  • Direct magnetic separation of red cells from whole blood

    Direct magnetic separation of red cells from whole blood Melville D Abstract THE magnetic properties of haemoglobin are well established 1 The prosthetic group of haemoglobin has a protoporphyrin structure ferrohaem in which the iron atom is ionically bound Consequently there are in deoxyhaemoglobin four unpaired electrons per iron atom

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  • Magnetophoretic Microseparators for Separating Blood Cells

    Direct magnetic separations of red blood cells from whole blood have been carried out using a continuous magnetic separation method based on high gradient magnetic separation HGMS and

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  • Continuous magnetic separation of blood components from

    Direct magnetic separations of red blood cells from whole blood have been carried out using a continuous magnetic separation method based on high gradient magnetic separation HGMS and a gaspermeable membrane with nitrogen gas

    Get Price
  • Continuous Magnetic Separation of Blood Components

    effective magnetic separation Direct magnetic separation of red and white blood cells from whole blood without any additives has been confirmed in a quasistatic state of veryFig 2 Fabricated continuous separator of 36 m flow length The flow channel low flow conditions using an optical observation m ethod 5 In those experiments the

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  • Optimized high gradient magnetic separation for isolation

    Feb 02 2010 Highly purified infected red blood cells irbc or highly synchronized parasite cultures are regularly required in malaria research Conventional isolation and synchronization rely on density and osmotic fragility of irbc respectively High gradient magnetic separation HGMS offers an alternative based on intrinsic magnetic properties of irbc avoiding exposure to chemicals and osmotic stress

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  • Design of microfluidic channels for magnetic separation of

    Feb 02 2016 Magnetic separation of parasitized malariainfected red blood cells within a parallelplate system was simulated using a CFDDEM EulerianLagrangian approach The plasma was treated as a Newtonian fluid as the Eulerian phase and the RBCs were treated as soft spheres as the Lagrangian phase influenced by drag collisions with other

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  • Direct magnetic separation of immune cells from whole

    Feb 05 2009 PBMCs comprise less than 005 of whole blood cells Usually PBMCs or leukocytes must be enriched from whole blood by densitygradient centrifugation for efficient cell separation Herein a magnetic cell separation system using protein GBacMPs was applied for the direct separation of immune cells from whole blood Figure 4B

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  • MACS174 Cell Separation

    Figure 12 MACSxpress Technology allows the isolation of cells from whole blood within 20 minutes 5 min 15 min Transfer and dilute sample Density gradient centrifugation without brake Layer density gradient medium Cell count Cell separation Magnetic labeling PBMC separation and wash Conventional protocol Transfer and dilute sample StraightFrom

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  • Magnetic cell separation Cell separation Miltenyi

    Immunomagnetic cell separation is based on antibodies coupled to magnetic beads During incubation with a cell suspension the antibodybead complex binds to cells expressing the corresponding epitope When the cell suspension is placed into a magnetic field magnetically labeled cells are retained while unlabeled cells can be removed

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  • Intrinsically magnetic susceptibility in human blood and

    Jul 01 2021 Monocytes are circulating blood cells that account for fewer than 10 of the total number of leukocytes Despite their low concentration in blood they play important roles in the immune system and their isolation with high purity is necessary for many research applicationsSome of the techniques used to separate them from other cells in the blood are magneticbased and rely on the

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  • Ultrahighthroughput magnetic sorting of large blood

    Jul 21 2020 circulating tumor cells microfluidics magnetic sorting leukapheresis liquid biopsy Approximately 90 of cancerrelated deaths are attributable to metastatic disease most commonly resulting from the bloodborne dissemination of circulating tumor cells CTCs 1 2CTCs are actively shed into the blood from primary and metastatic tumor deposits both as single cancer cells or as

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  • Direct magnetic separation of red cells from whole blood

    Jun 26 1975 Consequently red blood cells containing deoxyhaemoglobin when placed in a magnetic field B0 Tesla with field gradient d B0 d z may be expected to experience a magnetic force FM Newtons in the

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  • Magnetic Cell Separation Cell Isolation Technology

    Magnetic cell separation also known as immunomagnetic cell separation or magnetic cell sorting involves targeting cells for selection or depletion using antibodies or ligands directed against specific cell surface antigens Labeled cells are crosslinked to magnetic particles also known as magnetic beads that can be immobilized once an electromagnetic field is applied

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  • Magnetic Cell Separation and Depletion

    Magnetic Cell Separation and Depletion Isolation sorting andor enrichment of specific cell populations from blood tissues and other complex blends of biological material define a crucial step in biological research diagnostics therapeutics and other biomedical applications

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  • Magnetic Cell Separation and Depletion

    Magnetic Cell Separation and Depletion Isolation sorting andor enrichment of specific cell populations from blood tissues and other complex blends of biological material define a crucial step in biological research diagnostics therapeutics and other biomedical applications Cell separation can be achieved by a number of methods

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  • The basic guide to magnetic bead cell separation

    Magnetic cell separation entails the action of magne tic force on cellular particles blood bone marrow cultivation media food soil stool tissuehomogenates water and so forth set in solution Magnetic force applied to the solution separates treated cells

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  • Magnetic Separation Methods for the Detection of

    May 31 2017 PMS in combination with the phage method has been used to determine the viability of MAP cells in blood other magnetic separation methods have not been used in blood until now Similarly as in blood only one study using a magnetic separation method was conducted in cheese until now Here the IMS approach with automatic Pathatrix magnetic

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  • Magnetic Cell Separation Characterization of

    Nov 15 2003 Magnetic cell separation has become a popular technique to enrich or deplete cells of interest from a heterogeneous cell population One important aspect of magnetic cell separation is the degree to which a cell binds paramagnetic material It is this paramagnetic material that imparts a positive magnetophoretic mobility to the target cell thus allowing effective cell separation A

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  • Separation of red blood cells by magnetic means

    Oct 01 2000 A recent article by Haik et al is critically reviewed It is shown that the techniques of separation of red blood cells proposed by the above authors are unlikely to work and that highgradient magnetic separation is probably the only viable method of separation of erythrocytes

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  • Separation of red blood cells by magnetic means

    Oct 01 2000 The separation of erythrocytes red blood cells from the remainder of the blood components using magnetic means has received considerable attention during the last 20 years An innovative approach by a group of researchers from the Southampton University and other organisations 1 2 3 4 5 resulted in the development of a novel method of erythrocyte separation by highgradient magnetic separation

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  • Mixing Efficiency of Red Blood Cells With Magnetic

    Sep 16 2008 A hybrid separation system that utilizes centrifugation and magnetic separation is described Magnetic microspheres are utilized to isolate the red cells from the buffy coat on a continuous basis The effectiveness of red blood cell separation from the buffy coat in the hybrid system was improved over conventional centrifugal separation methods

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  • Microuidic high gradient magnetic cell separation

    The separation of red blood cells from whole blood by native susceptibility was shown in 1975 by Melville et al5 While most biological matter is composed of water a diamagnetic substance certain cells contain paramagnetic material The most paramagnetic cell in the body is the deoxygenated red blood cell Each red blood cell contains around

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